In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe (3)O (4) nanoparticles (NPs). The Fe (3)O (4) NPs were synthesized by coprecipitation followed by modification with ammonium oleate.

Magnetic immobilization is a novel approach for cell immobilization by using magnetic nanoparticles. ... Li YG, Gao HS, Li WL, Xing JM, Liu HZ (2009) In situ magnetic separation and immobilization of dibenzothiophene-desulfurizing bacteria. Bioresour Technol 100(21):5092–5096.

Field trials were conducted on April 13, 2019. Four treatments of the passivation measures were established as follows: (1) non‒BC, control (CK); (2) 7.5 t hm −2 BC, BCⅠ; (3) 15 t hm −2 BC, BCⅡ; and (4) 22.5 t hm −2 BC, BCⅢ. Using the randomized block design, each plot area was 20 m 2 (10 m × 2 m) and was replicated three times. …

This phenomenon is the basis for magnetic immobilization and magnetic separation. The potential of the nanoparticles to separate cells is affected by various parameters, such as the power of the external magnetic field, magnetic saturation value of the employed particles, nanoparticles zeta potential, time of exposure to the magnetic field, and ...

Europe PMC is an archive of life sciences journal literature. Do data resources managed by EMBL-EBI and our collaborators make a difference to your work? If so, please take 10 minutes to fill in our survey, and help us make the case for why sustaining open data resources is critical for life sciences research.

Magnetic nanocomposites have shown obvious advantages in enzyme immobilization due to their easy separation and recovery by magnetic fields, larger surface area and high mass transfer capacity. In this work, the research progress of magnetic nanocomposites as enzyme carriers is reviewed and the future development …

Magnetic separation technology is scalable and can be easily integrated in a recycle loop in a bioreactor to achieve a rapid recovery of bacterial clusters. ... Li YG, Gao HS, Li WL, Xing JM, Liu HZ (2009) In situ magnetic separation and immobilization of dibenzothiophene-desulfurizing bacteria. Bioresour Technol 100:5092–5096. Article …

In recent years, with advancements in nanotechnology and materials science, new enzyme immobilization strategies based on nanomaterials have continuously emerged. These strategies have shown significant effects on enhancing enzyme catalytic performance and stability due to their high surface area, good chemical stability, and …

Zero-valent iron (ZVI) facilitated in-situ selenium (Se) immobilization and its recovery by magnetic separation: Mechanisms and implications for microbial ecology. Zhongli Wang Changwei Lü Yanming Wang Rachel L. …

Raspberry-like colloidal polymer/magnetite/silica composite microspheres were rationally fabricated based on in situ growth or interfacial immobilization of magnetic nanoparticles (MNPs) onto the polymer …

Besides, a magnetic-MOF that is more convenient for recycling was developed by introducing magnetic moieties to allow magnetic separation from reaction mixtures (Huo et al., 2015; ... The immobilization strategies such as surface immobilization, in situ approach, covalent linkage and pore entrapment have been …

In summary, we created an enzyme-cell co-immobilization platform for scalable fabrication of highly efficient biocatalysts via facile in situ assembly of cells and …

Enhanced reduction and in-situ stabilization of Cr ... Schematic diagram of the reduction–adsorption and immobilization processes by magnetic PDA contained in SMP. 4. ... Excellent Cr(VI) capture capacity and magnetic separation were verified, and the interaction mechanisms were proposed in detail, based on a variety of methods. Ion …

Here we describe magnetic immobilization as a promising and novel technique for cell immobilization by using magnetic nanoparticles. This technique is based on the decoration of cells with magnetic nanoparticles to make them sensitive to magnetic field. ... Xing JM, Liu HZ (2009) In situ magnetic separation and immobilization of ...

A new and simple method has been proposed to prepare magnetic Fe(3)O(4)-chitosan (CS) nanoparticles by cross-linking with sodium tripolyphosphate (TPP), precipitation with NaOH and oxidation with O(2) in hydrochloric acid aqueous phase containing CS and Fe(OH)(2), and these magnetic CS nanoparticles …

The in-situ synthesis process proved to be useful for the preparation of MOF composite carriers [35].Magnetic chitosan (CS) precipitates can be formed by dropping Fe 3 O 4-containing chitosan acetic acid solution into NaOH solution and continuously stirring gently during aging.When Zn(NO 3) 2 ·6H 2 O is added to the chitosan solution, the affinity of …

In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe 3 O 4 nanoparticles (NPs). The Fe 3 O 4 NPs were synthesized by coprecipitation followed by modification with ammonium oleate. The surface-modified NPs were monodispersed and the particle size was about 13nm with …

This investigation may help introduce magnetic protein into biotechnology applications, and the one-step purification and immobilization method may serve to illustrate an economically viable ...

The magnetic chitosan nanocomposites have been studied intensively and been used practically in various biomedical and biological applications including enzyme immobilization. However, the loading capacity and the remained activity of immobilized enzyme based on existing approaches are not satisfied. Simpler and more effective …

Here, we present a novel technique to immobilize magnetic particles onto whole G. oxydans in situ via a synthetic adhesive biomimetic material inspired by the protein glues of marine mussels. Our approach involves simple coating of a cell adherent polydopamine film onto magnetic nanoparticles, followed by conjugation of the polydopamine-coated …

Enzyme Engineering for In Situ Immobilization Molecules. 2016 Oct 14;21(10):1370. doi: 10.3390/molecules21101370. Authors Fabian B H Rehm 1, Shuxiong Chen 2, Bernd H A Rehm 3 Affiliations 1 Institute of Fundamental Sciences, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand. …

In situ magnetic separation and immobilization of dibenzothiophene-desulfurizing bacteria. Yu-guang Li Hong Gao Wang-liang Li J. Xing ... It could be demonstrated that protease yield in total was enhanced by using in situ magnetic separation, and degradation of the protease, which followed first order kinetics, was reduced by using …

Request PDF | In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution | A new and simple method has been proposed ...

Europe PMC is an archive of life sciences journal literature.

In addition, recent studies showed that remediation by in situ immobilization could be avoided by taking advantage of the strong magnetic characteristics of ZVI. We combined these well-established concepts and conducted laboratory experiments to predict the removal efficiency of metals from contaminated soils based on their chemical ...

In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe(3)O(4) nanoparticles (NPs). The …

Immobilization of bacteriophages onto solid supports such as magnetic particles has demonstrated ultralow detection limits as biosensors for the separation and detection of their host bacteria. While the potential impact of magnetized phages is high, the current methods of immobilization are either weak, costly, inefficient, or laborious …

In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe 3 O 4 nanoparticles (NPs). The Fe 3 O 4 NPs were synthesized by coprecipitation followed by modification with ammonium oleate. The surface-modified NPs were monodispersed and the particle size was about 13 nm with …

The 16 s rRNA sequencing revealed the variations in the microbial communities in response to ZVI and Se, which the magnetic separation could potentially mitigate in the long term. This study provides a novel technique to achieve in-situ Se remediation and recovery by combining ZVI reduction and magnetic separation.

Request PDF | On May 1, 2024, Zhongli Wang and others published Zero-valent iron (ZVI) facilitated in-situ selenium (Se) immobilization and its recovery by magnetic separation: mechanisms and ...